Manual and Instructions
The Directorate of Forensic Sciences,
Ministry of Home Affairs, Govt. of India has prepared and published laboratory
procedure manual in various discipline of Forensic Science in the year-2005.
These manuals have been circulated to all the State and Central Laboratory in
the India so as to have uniformity in examination and reports. We are following
the same laboratory manual procedures alongwith other published protocols in
Forensic Sciences in the State Forensic Science Laboratory in HP.
It gives the
protocols for the examination of:-
- Examination
of blood and blood stains.
- Examination
of other fluids and the stains.
- Hair
and fibre Examination.
- Histogical
examination of tissues.
- Identification
of Food stuffs and stains.
- Analysis
of paper.
- Poisonous
plants.
- Identification
of wood.
- Plants
and their derivatives.
ii.) Forensic
Serology Manual:
It contains the protocols for the search, sampling and
storage procedure of serological samples, procedure for species of origin test,
alongwith blood grouping, Isogyme and paternity testing and the statistical
methods for the calculation of Gene frequency and paternity index.
Note:- For Biological
and Serological examinations Methods Manual of Metropolitan Police Forensic
Science. Biology Laboratory, London is also consulted.
iii.) Forensic
Chemistry Manual:
It gives the
protocols for the examination of cement, mortar, concrete fires and arson scene
residues.
- Oil
and fats.
- Analysis
of gold and other metals in cheating cases.
- Acid
and alkalies.
- Examination
of chemicals used in trap cases.
- Analysis
in liquors/ drinks.
- Soaps
and detergent and other miscellaneous items.
Manual of petroleum products-It gives the protocol for the examination of petroleum
products.
- Motor
gasoline/motor spirit/petrol.
- Kerosene
/ super kerosene oil (SKO).
- Diesel
(light diesel oil and high speed diesel),
- Aviation
turbine fuel (ATF Kerosene).
- Engine
lubricating oils.
- Grease.
- Furnace
oils.
- It
contains the methods for the analysis of poisons and poisoning isolation and purification
of poisons.
- Analysis
of inorganic poison (cations and anions)
- Analysis
of natural poison ( organic non-volatiles)
- Analysis
of acidic (organic non-volatiles poisons/drugs).
- Analysis
of miscellaneous substances.
- Instrumental
analysis in Toxicology.
iv.) Forensic
Physics Manual: It gives the procedure for the examination of
foot/foot wear/tyres impressions.
- Feature
of Tool Marks.
- Examination
of Tampered electronic energy meters.
- Identification
of cameras from film negatives.
- Examination
of paint.
- Examination
of identification marks.
- Examination
of glass fragments voice analysis.
- Examination
of soil samples.
- Examination
of concrete.
- Forensic
Fiber Examination.
- Examination
of broken objects.
- Forensic
Examination of Electrical cables/ wires.
- Forensic
Examination of papers.
v.) Forensic
Ballistics Manual:
This manual contains the genera; procedure for the receipt’ opening, marking,
examination and reporting procedures of the Forensic Ballistics exhibits. The
examination procedures on the following aspects are contained in the manual
alongwith requirements and safety aspects. The examination
procedures have been prescribed for :-
- Barrel
wash examination.
- Examination
of firearm or parts, thereof manufacturing tool.
- Examination
of firearm, prone to accidental discharge.
- Identification
of firearm type, and model.
- Examination
of firearm, whether its parts tempered with.
- Restoration
of Erased marking on firearm.
- Examination
of ammunition or parts thereof / manufacturing Tools.
- Identification
of ammunition/ parts thereof for type, make, and calibrc.
- Identification
of Bullet/ slug / parts thereof.
- Identification
of shots and pellets.
- Identification
of wads/ wad pieces.
- Identification
of propellant change.
- Linkage
of fired/ misfired cartridge.
- Linkage
of Fired Bullet/ Bullet Fragments with Rifled firearm.
- Bullets/
Pallets/ wad whether fired or not.
- Examination
of Target involved in shooting.
- Measurement
of wound ballistic parameters.
- Chemical
tests.
- Test
firing.
- Test
specimen.
- Automatic
examination and comparison of fired bullets/ cartridge cases and creation of a
national Database.
vi.) Forensic
Document examination Manual: It gives the general principles of
identification:
- Basis
of Handwriting identification.
- Detection
of forgery and fixing the authorship of forged signatures.
- Examination
type-script.
- Determination
of Sequence of strokes in Forensic Document Problems.
- Examination
of other physical evidences like folds and creases, staples; alpin marks etc.
- Identification
of printed matter, computer print-outs and other Mechanical impressions.
- Examination
of credit cards and travel documents.
- Forensic
Examination of writing materials i.e. inks and paper.
- Indentations,
alterations, erasures, additions, substitution and obliteration in Forensic Documents.
- List
of standard reference books and journals in Forensic Documents containing the
methods and techniques of analysis as applicable in forensic
Documents.
Laboratory Procedure Manuals: For examination of counterfeit or forged
currency/ bank
notes and other related exhibits -2004.
The
instructions for IOs for forwarding of cases.
- Report
case in the laboratory.
- Laboratory
Examination.
- Examination
of security features.
- Instrumental
techniques.
- Case
opening sheet.
- Work/observation
sheet.
- Examination
report programme.
vii.) Forensic
photography: It
is done by Forensic methods published in the scientific literature Indoor
photography for recording the forensic findings in the laboratory and out door
photography at the scenes of crime is done to record the forensic evidence
present at the SOC. The negatives of the photographs are taken and
preserved in the case files of the laboratory. The photographs are
submitted to the courts of law whenever required.
viii.) DNA Examination
Manual: This
manual contains the genera, procedure for the receipt, opening, marking,
examination and reporting procedures of the DNA exhibits. The examination
procedures on the following aspects are contained in the manual alongwith
requirements and safety aspects:
- DNA
isolation from Biospecimen.
- Quantitation
of DNA.
- STR
Typing by Automated DNA sequencer.
- Mitochondrial
DNA Analysis.
- Y
– STR Analysis.
- Identification
of Wildlife Species.
- Statistical
Analysis.
- Quality
Assurance Standards.
- Reagent
Preparation
ix.) Narcotic
Drugs & Psychotrophic Substances Manual:
This manual contains the genera, procedure for the
receipt, opening, marking, examination and reporting procedures of the NDPS
exhibits. The examination procedures on the following aspects are contained in
the manual alongwith requirements and safety aspects
Identification and examination of:-
- Opium/crude
morphine/poppy straw.
- Diacetyl
morphine/Heroin/Smack/Brown Sugar.
- Cannabis
- Methaqualone/Mecloqualone.
- Amphetamines/Methamphetamines.
- Barbiturates/barbiturate
Derivatives.
- Benzodiazepines.
- Mescaline.
- Psilocin/Psilocybin.
- Lysergide
(LSD)
- Miscellaneous drugs.